Purified antibody

Based on this purified antibody , an indirect competitive ELISA was developed .

以此抗体为基础，设计了间接竞争两步酶联免疫检测方法，并优化了缓冲液浓度、pH、吐温20浓度等参数。

The purified antibody was used to coat 96 hole board as capture antibody .

采用纯化的抗体包被96孔板，形成固相抗体。

The purified antibody could stimulate the proliferation of XG-2 cell line .

纯化后的mAb对XG-2细胞有明显的促增殖作用。

Colloidal gold was prepared and coupled with one of the purified antibody proteins so that the immuno - colloidal gold was obtained .

制备胶体金并用一纯化的单克隆抗体包被，制成免疫胶体金。

Western blot experiments indicated that the partially purified antibody preparations reacted specifically with the PKS proteins expressed in E. coli and , as expected , also with the very large PKS proteins naturally present in S. sp. FR-008 .

蛋白质印迹实验显示，部分提纯的抗体样品与大肠杆菌中表达的PKS蛋白有特异性反应。同时，这些抗体也可与天然存在于链霉菌FR-008的、巨大的PKS蛋白特异性地反应。

The specificity of the purified polyclonal antibody was identified by indirect fluorescence staining and FCM analysis .

间接免疫荧光染色及流式细胞术鉴定多克隆抗体的特异性。

The results showed that the recovery rates of purified monoclonal antibody were higher by using ammonium sulfate precipitation and caprylic acid-ammonium method than that by using protein A chromatography .

结果表明，纯化后单抗的回收率以硫酸铵沉淀法及辛酸-硫酸铵法较高，硫酸铵-蛋白A亲和层析法较低；

Plasma ACTH was measured by a solid phase RIA with a purified second antibody ( Ab2 ) immobilized on polystyrene tubes coated with styrene maleic anhydride copolymer .

采用自制马来酸酐－苯乙烯共聚物活化管，将二抗固定在聚苯乙烯管壁上，利用ACTH抗血清与固化二抗结合反应，制备ACTH固抗管，建立了测定血浆ACTH的固相放射免疫分析。

The purified monoclonal antibody completely lost its activity when heated at 56 ℃ for 5 minutes , but the activity of crude monoclonal antibody was stable even heated for 30 minutes at 56 ℃ .

纯化单克隆抗体在56℃加热5min，即失去抗体活性，而未经纯化的单克隆抗体即使加热30min，抗体活性也毫无损失。

Moreover , a high titer ( 1:32 ) rabbit antiserum against porcine plasma FN ( free from PN ) was prepared from immunized rabbit , from which affinity purified anti-FN antibody was obtained through FN-Sepharose 4B column .

此外，用无PN的FN免疫兔，制得高效价FN抗血清（1：32），并进一步纯化制得亲和层析纯FN抗体。

The Body Fluid Purified Protein Derivative Antibody Tests : Diagnosis Value in Pediatric Tuberculosis

结核菌素抗体对儿童结核病的诊断价值

Clinical significance of anti tuberculin purified protein derivative antibody assay in the diagnosis of children tuberculosis

测定血清抗结核菌素纯蛋白衍生物IgG诊断小儿肺结核的临床意义

Avian reticuloendotheliosis virus ( REV ) was purified and monoclonal antibody against REV was used . A modified bloking ELISA was developed for the detection of antibody in chicken sera against REV.36 serum samples collected randomly from chicken flocks in Beijing were tested by the blocking ELISA .

应用禽网状内皮组织增殖病病毒纯化抗原和抗REV单克隆抗体建立了改良阻断ELISA用于鸡血清中REV抗体检测，并对北京地区鸡群中随机采样的36份血清样本进行了检测，阳性率为5.6%。

Meanwhile , polyclonal antibody was purified by affinity chromatography and ammonium sulfate precipitation in this article and the antibody titer was detected by indirect ELISA . The purified antibody titers were respectively 1:16000 and 1:32000 .

同时，通过亲和层析法和饱和硫酸铵法纯化的抗体经间接ELISA法检测，其效价分别为1：16000和1：32000。